Enzyme Kinetics And Mechanism

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Author: Paul Cook
Publisher: Garland Science
ISBN: 1136844287
Size: 59.70 MB
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Enzyme Kinetics And Mechanism by Paul Cook


Original Title: Enzyme Kinetics And Mechanism

Enzyme Kinetics and Mechanis is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism‹including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Contemporary Enzyme Kinetics And Mechanism

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Author:
Publisher: Academic Press
ISBN: 9780123847447
Size: 71.31 MB
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Contemporary Enzyme Kinetics And Mechanism by


Original Title: Contemporary Enzyme Kinetics And Mechanism

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Contemporary Enzyme Kinetics And Mechanism

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Author: Daniel L. Purich
Publisher:
ISBN:
Size: 55.32 MB
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Contemporary Enzyme Kinetics And Mechanism by Daniel L. Purich


Original Title: Contemporary Enzyme Kinetics And Mechanism

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, and 249 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Key Features * Transient kinetic approaches to enzyme mechanisms * Designing initial rate enzyme assay * Deriving initial velocity and isotope exchange rate equations * Plotting and statistical methods for analyzing rate data * Cooperativity in enzyme function * Reversible enzyme inhibitors as mechanistic probes * Transition-state and multisubstrate inhibitors * Affinity labeling to probe enzyme structure and function * Mechanism-based enzyme inactivators * Isotope exchange methods for elucidating enzymatic catalysis * Kinetic isotope effects in enzyme catalysis * Site-directed mutagenesis in studies of enzyme catalysis

Enzyme Kinetics And Mechanism Part F Detection And Characterization Of Enzyme Reaction Intermediates

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Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 9780080489384
Size: 58.90 MB
Format: PDF, Kindle
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Enzyme Kinetics And Mechanism Part F Detection And Characterization Of Enzyme Reaction Intermediates by Daniel L. Purich


Original Title: Enzyme Kinetics And Mechanism Part F Detection And Characterization Of Enzyme Reaction Intermediates

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates

Enzyme Kinetics And Mechanism

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Author: Vern L. Schramm
Publisher: Elsevier
ISBN: 0121822095
Size: 41.12 MB
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Enzyme Kinetics And Mechanism by Vern L. Schramm


Original Title: Enzyme Kinetics And Mechanism

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Enzyme Kinetics Catalysis And Control

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Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0123809258
Size: 29.56 MB
Format: PDF, ePub, Mobi
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Enzyme Kinetics Catalysis And Control by Daniel L. Purich


Original Title: Enzyme Kinetics Catalysis And Control

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Enzyme Kinetics And Mechanisms Part E Energetics Of Enzyme Catalysis

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Author:
Publisher: Elsevier
ISBN: 0080496660
Size: 23.78 MB
Format: PDF, ePub, Docs
View: 2038

Enzyme Kinetics And Mechanisms Part E Energetics Of Enzyme Catalysis by


Original Title: Enzyme Kinetics And Mechanisms Part E Energetics Of Enzyme Catalysis

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

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