Synthetic Biology Part B

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Author: Chris Voigt
Publisher: Academic Press
ISBN: 0123851211
Size: 22.12 MB
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Synthetic Biology Part B by Chris Voigt


Original Title: Synthetic Biology Part B

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Dna Microarrays Part B Databases And Statistics

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Publisher: Elsevier
ISBN: 0080464661
Size: 13.45 MB
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Dna Microarrays Part B Databases And Statistics by


Original Title: Dna Microarrays Part B Databases And Statistics

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Mitochondrial Function

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Publisher: Academic Press
ISBN: 0128016159
Size: 48.42 MB
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Mitochondrial Function by


Original Title: Mitochondrial Function

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Provides invaluable details on state-of-the-art methods to assess a broad array of mitochondrial functions

Methods In Methane Metabolism Part B

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Author: Amy Rosenzweig
Publisher: Academic Press
ISBN: 0123869064
Size: 78.63 MB
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Methods In Methane Metabolism Part B by Amy Rosenzweig


Original Title: Methods In Methane Metabolism Part B

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Cryo Em Part B 3 D Reconstruction

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Publisher: Academic Press
ISBN: 9780123849922
Size: 75.90 MB
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Cryo Em Part B 3 D Reconstruction by


Original Title: Cryo Em Part B 3 D Reconstruction

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Guide To Techniques In Mouse Development Part B

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Author: Paul Wassarman
Publisher: Academic Press
ISBN: 9780123848819
Size: 65.35 MB
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Guide To Techniques In Mouse Development Part B by Paul Wassarman


Original Title: Guide To Techniques In Mouse Development Part B

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

Imaging And Spectroscopic Analysis Of Living Cells

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Author: P. Michael Conn
Publisher: Academic Press
ISBN: 0123918561
Size: 36.91 MB
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Imaging And Spectroscopic Analysis Of Living Cells by P. Michael Conn


Original Title: Imaging And Spectroscopic Analysis Of Living Cells

Going back to the dawn of light microscopy, imaging techniques have provided the opportunity for developing models of cellular function. Over the past 40 years, the availability of technology for high-resolution imaging and for evaluation of those images in live cells has extended our reach and, accordingly, our ability to understand cell function. Given the large number of choices in equipment and approaches, sorting out the best approach can be challenging, even to seasoned investigators. The present volumes provide descriptions of methods used to image living cells, with particular reference to the technical approaches and reagents needed and approaches to selecting the best techniques. The authors explain how these methods are able to provide important biological insights in normal and pathological cells. Authors were selected based on research contributions in the area about which they have written and based on their ability to describe their methodological contribution in a clear and reproducible way. They have been encouraged to make use of graphics, comparisons to other methods, and to provide tricks and approaches not revealed in prior publications that make it possible to adapt methods to other systems

Imaging And Spectroscopic Analysis Of Living Cells

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Publisher: Academic Press
ISBN: 012391907X
Size: 12.85 MB
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Imaging And Spectroscopic Analysis Of Living Cells by


Original Title: Imaging And Spectroscopic Analysis Of Living Cells

This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods

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