Membrane Protein Purification And Crystallization

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Author: Carola Hunte
Publisher: Elsevier
ISBN: 0080536174
Size: 10.64 MB
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Membrane Protein Purification And Crystallization by Carola Hunte

Original Title: Membrane Protein Purification And Crystallization

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

Introduction To Experimental Biophysics Second Edition

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Author: Jay L. Nadeau
Publisher: CRC Press
ISBN: 1351000926
Size: 39.75 MB
Format: PDF, Kindle
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Introduction To Experimental Biophysics Second Edition by Jay L. Nadeau

Original Title: Introduction To Experimental Biophysics Second Edition

Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ?Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.

Membrane Proteins Engineering Purification And Crystallization

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Publisher: Academic Press
ISBN: 0128021934
Size: 75.95 MB
Format: PDF
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Membrane Proteins Engineering Purification And Crystallization by

Original Title: Membrane Proteins Engineering Purification And Crystallization

Membrane Proteins – Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In Enzymology Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Mitochondrial Function Part A

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Publisher: Academic Press
ISBN: 0080915337
Size: 35.32 MB
Format: PDF, ePub, Mobi
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Mitochondrial Function Part A by

Original Title: Mitochondrial Function Part A

The first of two new volumes covering mitochondria, Mitochondrial Function, Part A presents modern methods that have been developed to examine mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species. These new techniques provide investigators with sensitive, original approaches to the study of disease states associated with mitochondrial malfunction. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them Along with companion volume covering mitochondrial diseases and defects, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Molecular Bioelectronics

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Author: Nicolini Claudio
Publisher: World Scientific
ISBN: 9814725862
Size: 76.13 MB
Format: PDF, Kindle
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Molecular Bioelectronics by Nicolini Claudio

Original Title: Molecular Bioelectronics

Molecular bioelectronics is a field in strong evolution at the frontier of life and materials sciences. The term is utilized in a broad context to emphasize a unique blend of electronics and biotechnology which is seen as the best way to achieve many objectives of industrial and scientific relevance, including biomolecular engineering, bioelectronic devices, materials and sensors capable of optimal hardware efficiency and intelligence and molecular miniaturization. Contents: IntroductionActive BioelementsTechnologiesBioelectronic MaterialsBioelectronic SensorsBioelectronic Molecular DevicesProtein AutomataConclusionReferences Readership: Students and scientists in bioelectronics and materials science.

Atomic Force Microscopy For Biologists

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Author: Victor J Morris
Publisher: World Scientific
ISBN: 190897821X
Size: 56.94 MB
Format: PDF
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Atomic Force Microscopy For Biologists by Victor J Morris

Original Title: Atomic Force Microscopy For Biologists

Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the "natural" conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems. The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike. Contents:ApparatusBasic PrinciplesMacromoleculesInterfacial SystemsOrdered MacromoleculesCells, Tissue and BiomineralsOther Probe MicroscopesForce Spectroscopy Readership: Undergraduates, postgraduates and researchers in biophysics. Keywords:AFM;Scanning Probe Microscopy (SPM);Biophysics;Cells;Macromolecules;Imaging;Biopolymers;Interfaces;DNA;PhospholipidsKey Features:Over 70 new pages, 700 new references, bringing it bang up-to-date with current literature, and 24 new figuresOnly book covering published research, machines and methodology from inception to the present day (2009)Updated to include and an entirely new chapter on force spectroscopyReviews:“This second edition of an excellent book updates considerably the information contained, and is expanded, too. The main focus of this book are the biological applications of AFM, and these are covered very well … The chapters are in-depth and very informative, and contain lots of useful and detailed information. In general the book is well written with an informal style, and contains useful information for beginners, including detailed information on how to carry out some experiments, answers to common questions, etc. … Overall this book is highly recommended for those wishing to get an overview of the biological applications of AFM.”Peter Eaton University of Porto, Portugal Reviews of the First Edition “This book fills an important niche by providing an introduction to AFM that will be understandable to a wide range of scientists … the reader is offered an introduction of sufficient brevity to actually be read and of sufficient depth to help in defining the potential uses of SPMs in his or her own research.” Scott Fraser (California Institute of Technology, USA) Nature Cell Biology “I found this book easy to read with clear, simple explanations of a complex topic. I think that the selection of the subjects treated in the book is sufficient for the non-specialist to understand both the basic concepts of the AFM and the production of AFM images in biological systems.”Microscopy and Analysis “… is an excellent introduction for anybody wishing to enter this field … In summary I would strongly recommend this book to any biologist planning on carrying out research using SPM imaging techniques and existing users in the field who wish to broaden their knowledge of SPM imaging and the research already carried out on common biological systems.”Drew Murray JPK Instruments, Berlin “This book provides an excellent survey of novel applications. It is nicely illustrated with numerous images from leading experts in the field. Clear descriptions of the apparatus and its basic principles are provided in a way accessible to students/scientists who do not have a strong physics background. It will be useful to biologists, but also non-biologists dealing with biosystems …”Prof Y F Dufrêne UCL, Belgium

Purification Of Laboratory Chemicals

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Author: W.L.F. Armarego
Publisher: Butterworth-Heinemann
ISBN: 0128054565
Size: 65.16 MB
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Purification Of Laboratory Chemicals by W.L.F. Armarego

Original Title: Purification Of Laboratory Chemicals

Purification of Laboratory Chemicals, Eighth Edition, tabulates methods taken from literature for purifying thousands of individual commercially available chemicals. To help in applying this information, the more common processes currently used for purification in chemical laboratories and new methods are discussed. For dealing with substances not separately listed, a chapter is included setting out the usual methods for purifying specific classes of compounds. Features empirical formulae inserted for every entry References all important applications of each substance Updates and confirms the accuracy of all CAS registry numbers, molecular weights, original reference, and physical data Provides increased coverage of the latest commercial chemical products, including pharmaceutical chemicals, updated safety and hazard material, and expanded coverage of laboratory and work practices and purification methods

Filtration And Purification In The Biopharmaceutical Industry Second Edition

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Author: Maik J. Jornitz
Publisher: CRC Press
ISBN: 9780849379543
Size: 51.20 MB
Format: PDF
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Filtration And Purification In The Biopharmaceutical Industry Second Edition by Maik J. Jornitz

Original Title: Filtration And Purification In The Biopharmaceutical Industry Second Edition

Filtration and Purification in the Biopharmaceutical Industry, First Edition greatly expands its focus with extensive new material on the critical role of purification and the significant advances in filtration science and technology. This new edition provides state-of-the-science information on all aspects of filtration and purification, including the current methods, processes, technologies and equipment, and brings you up-to-date with the latest industry standards and regulatory requirements for the pharmaceutical and biopharmaceutical industries. An essential, comprehensive, source for all professionals involved with filtration and purification practices and compliance, this text · addresses recent biotechnology-related processes and advanced technologies, such as viral retentive filters, membrane chromatography, downstream processing, cell harvesting, and medium and buffer filtration · presents detailed updates on the latest FDA and EMEA regulatory requirements involving filtration and purification practices · describes current industry quality standards and validation requirements and provides guidance for compliance Audience:

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