Membrane Protein Purification And Crystallization

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Author: Carola Hunte
Publisher: Elsevier
ISBN: 0080536174
Size: 57.64 MB
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Membrane Protein Purification And Crystallization by Carola Hunte


Original Title: Membrane Protein Purification And Crystallization

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

The Next Generation In Membrane Protein Structure Determination

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Author: Isabel Moraes
Publisher: Springer
ISBN: 3319350722
Size: 60.32 MB
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The Next Generation In Membrane Protein Structure Determination by Isabel Moraes


Original Title: The Next Generation In Membrane Protein Structure Determination

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

Introduction To Experimental Biophysics

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Author: Jay Nadeau
Publisher: CRC Press
ISBN: 1439897409
Size: 79.92 MB
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Introduction To Experimental Biophysics by Jay Nadeau


Original Title: Introduction To Experimental Biophysics

Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often specific to particular subject areas; nonetheless, there are a few basic methods that provide a conceptual underpinning for broad application. Introduction to Experimental Biophysics is the ideal benchtop companion for physical scientists interested in getting their hands wet. Assuming familiarity with basic physics and the scientific method but no previous background in biology or chemistry, this book provides: A thorough description of modern experimental and analytical techniques used in biological and biophysical research Practical information and step-by-step guidance on instrumentation and experimental design Recipes for common solutions and media, lists of important reagents, and a glossary of biological terms used Developed for graduate students in biomedical engineering, physics, chemical engineering, chemistry, mathematics, and computer science, Introduction to Experimental Biophysics is an essential resource for scientists to overcoming conceptual and technical barriers to working in a biology wet lab.

Membrane Protein Crystallization

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Author:
Publisher: Academic Press
ISBN: 9780080961590
Size: 52.24 MB
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Membrane Protein Crystallization by


Original Title: Membrane Protein Crystallization

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

A Practical Guide To Membrane Protein Purification

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Author: Gebhard von Jagow
Publisher: Elsevier
ISBN: 0080571727
Size: 43.81 MB
Format: PDF, Mobi
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A Practical Guide To Membrane Protein Purification by Gebhard von Jagow


Original Title: A Practical Guide To Membrane Protein Purification

A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Provides general guidelines and strategies for isolation of membrane proteins Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs Gives special emphasis to new native and denaturing electrophoresis techniques Explains modifications of techniques used for water-soluble proteins

Biochemical Engineering And Biotechnology

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Author: Ghasem Najafpour
Publisher: Elsevier
ISBN: 9780080468020
Size: 62.85 MB
Format: PDF
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Biochemical Engineering And Biotechnology by Ghasem Najafpour


Original Title: Biochemical Engineering And Biotechnology

Extensive application of bioprocesses has generated an expansion in biotechnological knowledge, generated by the application of biochemical engineering to biotechnology. Microorganisms produce alcohols and acetone that are used in industrial processes. The knowledge related to industrial microbiology has been revolutionized by the ability of genetically engineered cells to make many new products. Genetic engineering and gene mounting has been developed to enhance industrial fermentation. Ultimately, these bioprocesses have become a new way of developing commercial products. Biochemical Engineering and Biotechnology demonstrates the application of biological sciences in engineering with theoretical and practical aspects to enhance understanding of knowledge in this field. The book adopts a practical approach, showing related case studies with original research data. It is an ideal text book for college and university courses, which guides students through the lectures in a clear and well-illustrated manner. · Demonstrates the application of biological sciences in engineering with theoretical and practical aspects. · Unique practical approach, using case studies, detailed experiments, original research data and problems and possible solutions. · Gives detailed experiments with simple design equations and the required calculations.

Comprehensive Biotechnology

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Publisher: Newnes
ISBN: 0080885047
Size: 63.17 MB
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Comprehensive Biotechnology by


Original Title: Comprehensive Biotechnology

The second edition of Comprehensive Biotechnology continues the tradition of the first inclusive work on this dynamic field with up-to-date and essential entries on the principles and practice of biotechnology. The integration of the latest relevant science and industry practice with fundamental biotechnology concepts is presented with entries from internationally recognized world leaders in their given fields. With two volumes covering basic fundamentals, and four volumes of applications, from environmental biotechnology and safety to medical biotechnology and healthcare, this work serves the needs of newcomers as well as established experts combining the latest relevant science and industry practice in a manageable format. It is a multi-authored work, written by experts and vetted by a prestigious advisory board and group of volume editors who are biotechnology innovators and educators with international influence. All six volumes are published at the same time, not as a series; this is not a conventional encyclopedia but a symbiotic integration of brief articles on established topics and longer chapters on new emerging areas. Hyperlinks provide sources of extensive additional related information; material authored and edited by world-renown experts in all aspects of the broad multidisciplinary field of biotechnology Scope and nature of the work are vetted by a prestigious International Advisory Board including three Nobel laureates Each article carries a glossary and a professional summary of the authors indicating their appropriate credentials An extensive index for the entire publication gives a complete list of the many topics treated in the increasingly expanding field

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