Microbial Growth In Biofilms

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Author: Ronald J. Doyle
Publisher: Gulf Professional Publishing
ISBN: 9780121822385
Size: 49.82 MB
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Microbial Growth In Biofilms by Ronald J. Doyle


Original Title: Microbial Growth In Biofilms

This volume and its companion, Volume 337, supplement Volume 310. These volumes provide a contemporary sourcebook for virtually any kind of experimental approach involving biofilms. They cover bioengineering, molecular, genetic, microscopic, chemical, and physical methods.

Complex Enzymes In Microbial Natural Product Biosynthesis Part B Polyketides Aminocoumarins And Carbohydrates

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Publisher: Academic Press
ISBN: 9780080923369
Size: 40.68 MB
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Complex Enzymes In Microbial Natural Product Biosynthesis Part B Polyketides Aminocoumarins And Carbohydrates by


Original Title: Complex Enzymes In Microbial Natural Product Biosynthesis Part B Polyketides Aminocoumarins And Carbohydrates

Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. * Gathers tried and tested methods and techniques from top players in the field. * Provides an extremely useful reference for the experienced research scientist. * Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates

Synthetic Biology Part B

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Author: Chris Voigt
Publisher: Academic Press
ISBN: 0123851211
Size: 62.13 MB
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Synthetic Biology Part B by Chris Voigt


Original Title: Synthetic Biology Part B

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation

Mitochondrial Function Part B

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Publisher: Academic Press
ISBN: 9780080923505
Size: 76.80 MB
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Mitochondrial Function Part B by


Original Title: Mitochondrial Function Part B

In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them Along with a companion volume covering topics including mitochondrial electron transport chain complexes and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Recognition Of Carbohydrates In Biological Systems Part B Specific Applications

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Publisher: Elsevier
ISBN: 0080497063
Size: 23.28 MB
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Recognition Of Carbohydrates In Biological Systems Part B Specific Applications by


Original Title: Recognition Of Carbohydrates In Biological Systems Part B Specific Applications

Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume, Recognition of Carbohydrates in Biological Systems, Part B: Specific Applications, and its companion, Volume 362, present state-of-the-art methodologies, as well as the most recent biological observations in this area. Covers carbohydrate-binding proteins Discusses glycoproteins and glycolipids Polysaccharides, enzymes and cells are also covered

Dna Microarrays Part B Databases And Statistics

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Publisher: Elsevier
ISBN: 0080464661
Size: 30.49 MB
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Dna Microarrays Part B Databases And Statistics by


Original Title: Dna Microarrays Part B Databases And Statistics

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Methods In Methane Metabolism Part B

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Author: Amy Rosenzweig
Publisher: Academic Press
ISBN: 0123869064
Size: 45.88 MB
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Methods In Methane Metabolism Part B by Amy Rosenzweig


Original Title: Methods In Methane Metabolism Part B

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Cryo Em Part B 3 D Reconstruction

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Publisher: Academic Press
ISBN: 9780123849922
Size: 72.19 MB
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Cryo Em Part B 3 D Reconstruction by


Original Title: Cryo Em Part B 3 D Reconstruction

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

Guide To Techniques In Mouse Development Part B

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Author: Paul Wassarman
Publisher: Academic Press
ISBN: 9780123848819
Size: 51.95 MB
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Guide To Techniques In Mouse Development Part B by Paul Wassarman


Original Title: Guide To Techniques In Mouse Development Part B

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods

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Publisher: Academic Press
ISBN: 9780123814838
Size: 19.29 MB
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Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods by


Original Title: Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter And Force Based Methods

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

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