Pcr Strategies

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Author: Michael A. Innis
Publisher: Elsevier
ISBN: 9780080538549
Size: 49.65 MB
Format: PDF
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Pcr Strategies by Michael A. Innis

Original Title: Pcr Strategies

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

Pcr Applications

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Author: Michael A. Innis
Publisher: Academic Press
ISBN: 9780080919638
Size: 14.87 MB
Format: PDF
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Pcr Applications by Michael A. Innis

Original Title: Pcr Applications

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Key Features * Focuses on gene discovery, genomics, and DNA array technology * Covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters * Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval

Pcr Troubleshooting And Optimization

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Author: Suzanne Kennedy
Publisher: Horizon Scientific Press
ISBN: 1904455727
Size: 17.50 MB
Format: PDF, Docs
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Pcr Troubleshooting And Optimization by Suzanne Kennedy

Original Title: Pcr Troubleshooting And Optimization

The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been employed. In addition, proper optimization of PCR conditions is required for the generation of specific, repeatable, reproducible, and sensitive data. This book discusses the strategies for preparing effective controls and standards for PCR, when they should be employed, and how to interpret the information they provide. It highlights the significance of optimization for efficiency, precision, and sensitivity of PCR methodology and provides essential guidance on how to troubleshoot inefficient reactions. Experts in PCR describe design and optimization techniques, discuss the use of appropriate controls, explain the significance of standard curves, and explore the principles and strategies required for effective troubleshooting. The book highlights the importance of sample preparation and quality, primer design, controlling inhibitors, avoiding amplicon and environmental contamination, optimizing reagent quality and concentration, and modifying the thermal cycling protocol for optimal sensitivity and specificity. In addition, specific chapters discuss the history of PCR, the choice of instrumentation, the applications of PCR in metagenomics, high resolution melting analysis, the MIQE guidelines, and PCR at the microliter scale. The strategies, tips and advice contained in this concise volume will enable the scientist to optimize and effectively troubleshoot a wide range of techniques, including PCR, reverse transcriptase PCR, real-time PCR, and quantitative PCR. It will be an essential book for anyone using PCR technology.

Pcr Cloning Protocols

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Author: Bing-Yuan Chen
Publisher: Springer Science & Business Media
ISBN: 1592591779
Size: 51.37 MB
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Pcr Cloning Protocols by Bing-Yuan Chen

Original Title: Pcr Cloning Protocols

Annotation PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Single Cell Diagnostics

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Author: Alan Thornhill
Publisher: Springer Science & Business Media
ISBN: 159745298X
Size: 33.63 MB
Format: PDF, ePub, Mobi
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Single Cell Diagnostics by Alan Thornhill

Original Title: Single Cell Diagnostics

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.

Pcr Protocols

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Author: John M. S. Bartlett
Publisher: Springer Science & Business Media
ISBN: 1592593844
Size: 35.38 MB
Format: PDF, ePub
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Pcr Protocols by John M. S. Bartlett

Original Title: Pcr Protocols

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly, its use has grown in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The methods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR technique in their work. 71 chapters of the most important PCR methodologies for your lab Includes the newest and most up-to-date collection for using PCR in a wide range of applications Provides an extensive range of versatile, expedient, and readily applicable PCR protocols Protocols are suitable for both novice and experienced researchers Notes section in each chapter provides tips, alternative suggestions, and other enhancements of the protocols.

Quantitative Real Time Pcr In Applied Microbiology

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Author: Martin Filion
Publisher: Horizon Scientific Press
ISBN: 1908230010
Size: 76.85 MB
Format: PDF, ePub
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Quantitative Real Time Pcr In Applied Microbiology by Martin Filion

Original Title: Quantitative Real Time Pcr In Applied Microbiology

Real time quantitative PCR (qPCR) technology has revolutionized almost all areas of microbiology, including clinical microbiology, food microbiology, industrial microbiology, environmental microbiology, and microbial biotechnology. Various modifications and improvements have enhanced the overall performance of this highly versatile technology and the qPCR instrumentation and strategies currently available are more sensitive, faster, and more affordable than ever before. Written by experts in the field and aimed specifically at microbiologists, this book describes and explains the most important aspects of current qPCR strategies, instrumentation, and software. Renowned scholars cover the application of qPCR technology in various areas of applied microbiology and comment on future trends. Topics include: instrumentation * fluorescent chemistries * quantification strategies * data analysis software * environmental microbiology * water microbiology * food microbiology * gene expression studies * validation of microbial microarray data * future trends in qPCR technology. This outstanding book will be invaluable for all microbiologists and is recommended for all microbiology laboratories.

Improvement Strategies Of Leguminosae Biotechnology

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Author: Pawan K. Jaiwal
Publisher: Springer Science & Business Media
ISBN: 9781402014055
Size: 36.59 MB
Format: PDF, ePub
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Improvement Strategies Of Leguminosae Biotechnology by Pawan K. Jaiwal

Original Title: Improvement Strategies Of Leguminosae Biotechnology

Legumes include many very important crop plants that contribute very critical protein to the diets of both humans and animals around the world. Their unique ability to fix atmospheric nitrogen in association with Rhizobia enriches soil fertility, and establishes the importance of their niche in agriculture. Divided into two volumes, this work presents an up-to-date analysis of in vitro and recombinant DNA technologies for the improvement of grain, forage and tree legumes. Volume 10A examines the current status and future prospects of challenges of the following: in vitro morphogenesis; biotic and abiotic stress tolerance; genomics; nitrogen fixation and utilization; nutritional improvement, and biodiversity of wild and tribal legumes. Volume 10B presents the current state and future prospects of in vitro regeneration and genetic transformation expression and stability of transgenes modification of traits in almost all the important legumes, for example: soybean; peanut; pea; french bean; chick pea; pigeon pea; cowpea; mung bean; black gram; azuki bean; lentil; Lathyrus; lupinus; Lotus spp; Medicago spp; Trifolium spp; Winged bean; Guar; and tree legumes for their improvement. Written by international experts, these volumes will be of great value to researchers, as well as graduate students and all those requiring an advanced level overview of the subject area.

Plant Molecular Biology Labfax

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Author: R. R. D. Croy
Publisher: Elsevier
ISBN: 9780121983703
Size: 36.65 MB
Format: PDF, Docs
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Plant Molecular Biology Labfax by R. R. D. Croy

Original Title: Plant Molecular Biology Labfax

LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogs, since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs. Plant Molecular Biology Labfax is a detailed compendium of essential information on plant nucleic acids, transformation and expression vectors, selectable genes and reporter genes, gene expression and PCR techniques, etc. A key feature is the Plant Gene Index, comprising comprehensive tables of plant genes published and submitted to sequence databases. Plant Molecular Biology Labfax, while specializing in molecular aspects of plant science, inevitably has numerous sections dealing with general molecular biology which complement the extensive information provided in Molecular Biology Labfax. It is therefore a worthy companion to this text.

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