Practical Protein Crystallography

Download Read Online Practical Protein Crystallography book
Author: Duncan E. McRee
Publisher: Academic Press
ISBN: 0323138888
Size: 78.15 MB
Format: PDF, Mobi
View: 7504

Practical Protein Crystallography by Duncan E. McRee


Original Title: Practical Protein Crystallography

Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.

Membrane Protein Purification And Crystallization

Download Read Online Membrane Protein Purification And Crystallization book
Author: Carola Hunte
Publisher: Elsevier
ISBN: 0080536174
Size: 39.15 MB
Format: PDF
View: 3560

Membrane Protein Purification And Crystallization by Carola Hunte


Original Title: Membrane Protein Purification And Crystallization

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

Crystallography Made Crystal Clear

Download Read Online Crystallography Made Crystal Clear book
Author: Gale Rhodes
Publisher: Elsevier
ISBN: 9780080455549
Size: 12.68 MB
Format: PDF, Kindle
View: 752

Crystallography Made Crystal Clear by Gale Rhodes


Original Title: Crystallography Made Crystal Clear

Crystallography Made Crystal Clear makes crystallography accessible to readers who have no prior knowledge of the field or its mathematical basis. This is the most comprehensive and concise reference for beginning Macromolecular crystallographers, written by a leading expert in the field. Rhodes' uses visual and geometric models to help readers understand the mathematics that form the basis of x-ray crystallography. He has invested a great deal of time and effort on World Wide Web tools for users of models, including beginning-level tutorials in molecular modeling on personal computers. Rhodes' personal CMCC Home Page also provides access to tools and links to resources discussed in the text. Most significantly, the final chapter introduces the reader to macromolecular modeling on personal computers-featuring SwissPdbViewer, a free, powerful modeling program now available for PC, Power Macintosh, and Unix computers. This updated and expanded new edition uses attractive four-color art, web tool access for further study, and concise language to explain the basis of X-ray crystallography, increasingly vital in today's research labs. * Helps readers to understand where models come from, so they don't use them blindly and inappropriately * Provides many visual and geometric models for understanding a largely mathematical method * Allows readers to judge whether recently published models are of sufficiently high quality and detail to be useful in their own work * Allows readers to study macromolecular structure independently and in an open-ended fashion on their own computers, without being limited to textbook or journals illustrations * Provides access to web tools in a format that will not go out of date. Links will be updated and added as existing resources change location or are added

Introduction To Macromolecular Crystallography

Download Read Online Introduction To Macromolecular Crystallography book
Author: Alexander McPherson
Publisher: John Wiley & Sons
ISBN: 1118210638
Size: 10.69 MB
Format: PDF, Kindle
View: 5674

Introduction To Macromolecular Crystallography by Alexander McPherson


Original Title: Introduction To Macromolecular Crystallography

A comprehensive and approachable introduction to crystallography — now updated in a valuable new edition The Second Edition of this well-received book continues to offer the most concise, authoritative, and easy-to-follow introduction to the field of crystallography. Dedicated to providing a complete, basic presentation of the subject that does not assume a background in physics or math, the book's content flows logically from basic principles to methods, such as those for solving phase problems, interpretation of Patterson maps and the difference Fourier method, the fundamental theory of diffraction and the properties of crystals, and applications in determining macromolecular structure. This new edition includes a vast amount of carefully updated materials, as well as two completely new chapters on recording and compiling X-ray data and growing crystals of proteins and other macromolecules. Richly illustrated throughout to clarify difficult concepts, this book takes a non-technical approach to crystallography that is ideal for professionals and graduate students in structural biology, biophysics, biochemistry, and molecular biology who are studying the subject for the first time.

Crystallization Of Nucleic Acids And Proteins

Download Read Online Crystallization Of Nucleic Acids And Proteins book
Author: Arnaud Ducruix
Publisher: Oxford University Press, USA
ISBN: 9780199636792
Size: 67.37 MB
Format: PDF, Mobi
View: 6727

Crystallization Of Nucleic Acids And Proteins by Arnaud Ducruix


Original Title: Crystallization Of Nucleic Acids And Proteins

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.

Crystallization Of Nucleic Acids And Proteins

Download Read Online Crystallization Of Nucleic Acids And Proteins book
Author: Arnaud Ducruix
Publisher: Oxford University Press
ISBN: 9780199632466
Size: 72.22 MB
Format: PDF, ePub, Docs
View: 6158

Crystallization Of Nucleic Acids And Proteins by Arnaud Ducruix


Original Title: Crystallization Of Nucleic Acids And Proteins

In the last decade, crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules. Other topics covered include: seeding procedures; crystallization in gels under microgravity and supergravity; crystallization of nucleic acids, nucleic acid-protein complexes and membrane proteins; introduction to X-ray crystallography; and automated processes. Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.

The Practice Of Medicinal Chemistry

Download Read Online The Practice Of Medicinal Chemistry book
Author: Camille Georges Wermuth
Publisher: Elsevier
ISBN: 0080497772
Size: 40.72 MB
Format: PDF
View: 5478

The Practice Of Medicinal Chemistry by Camille Georges Wermuth


Original Title: The Practice Of Medicinal Chemistry

The Practice of Medicinal Chemistry, 2E, is a single-volume source on the practical aspects of medicinal chemistry. The successful first edition was nicknamed "The Bible" by medicinal chemists, and the second edition has been updated, expanded and refocused to reflect developments over the last decade. Emphasis is put on how medicinal chemists conduct their search for and design of new drug entities. In contrast to competing books, it focuses on the chemistry rather than pharmacological concepts or descriptions of the various therapeutic classes of drugs. Most medicinal chemists working in the pharmaceutical industry are organic synthetic chemists who must acquire a strong knowledge of medicinal chemistry as they enter the industry. This book aims to be their practical handbook - a complete guide to the drug discovery process. * The only book available dealing with the practical aspects of medicinal chemistry * Serves as a complete guide to the drug discovery process, from conception of the molecules to drug production * Updated chapters devoted to the discovery of new lead compounds, including combinatorial chemistry

Principles Of Protein X Ray Crystallography

Download Read Online Principles Of Protein X Ray Crystallography book
Author: Jan Drenth
Publisher: Springer Science & Business Media
ISBN: 1475723350
Size: 28.90 MB
Format: PDF, ePub, Docs
View: 7148

Principles Of Protein X Ray Crystallography by Jan Drenth


Original Title: Principles Of Protein X Ray Crystallography

New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals so set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface Macromolecules are the principal nonaqueous components of living cells. Among the macromolecules (proteins, nucleic acids, and carbohydrates), proteins are the largest group.

Protein Crystallography

Download Read Online Protein Crystallography book
Author: Alexander Wlodawer
Publisher: Humana Press
ISBN: 9781493969982
Size: 64.20 MB
Format: PDF
View: 2512

Protein Crystallography by Alexander Wlodawer


Original Title: Protein Crystallography

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.

Recent Books :