Rna Protein Interactions A Practical Approach

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Author: Christopher W.J. Smith
Publisher: Oxford University Press, UK
ISBN: 0191591629
Size: 58.14 MB
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Rna Protein Interactions A Practical Approach by Christopher W.J. Smith

Original Title: Rna Protein Interactions A Practical Approach

RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -

A Laboratory Guide To Rna

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Author: Paul A. Krieg
Publisher: John Wiley & Sons
ISBN: 9780471125365
Size: 25.52 MB
Format: PDF
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A Laboratory Guide To Rna by Paul A. Krieg

Original Title: A Laboratory Guide To Rna

Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques. Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips. A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms. Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.

Multiplication Of Rna Plant Viruses

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Author: Chundi L. Mandahar
Publisher: Springer Science & Business Media
ISBN: 1402047258
Size: 41.15 MB
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Multiplication Of Rna Plant Viruses by Chundi L. Mandahar

Original Title: Multiplication Of Rna Plant Viruses

Biochemical studies on plant virus RNA replication have advanced considerably since 2000, primarily because of new genetic, molecular, biochemical, and enzymatic studies. This book generates understanding of multiplication of plus-sense RNA plant viruses, especially at molecular level. Certain virus-encoded essential proteins, nucleotide sequence motifs, and RNA secondary structures are central to virus RNA replication, which has a number of stages. Each is a complex phenomenon requiring specific factors and conditions.

Rna And The Regulation Of Gene Expression

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Author: Kevin V. Morris
Publisher: Horizon Scientific Press
ISBN: 1904455255
Size: 53.73 MB
Format: PDF, ePub
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Rna And The Regulation Of Gene Expression by Kevin V. Morris

Original Title: Rna And The Regulation Of Gene Expression

The role of RNA in regulating gene expression has become a topic of intense interest. In this book, internationally recognized experts in RNA research explore and discuss the methods whereby RNA can regulate gene expression with examples in yeast, Drosophila, mammals, and viral infection, as well as highlight the application of this knowledge in therapeutics and research. Topics include: gene silencing and gene activation, the hammerhead ribozyme, epigenetic regulation, RNAi, microRNA, and pyknons. This comprehensive publication is intended for readers with teaching or research interests in RNA, the regulation of gene expression, genetics, genomics, and molecular biology.

Rna Turnover In Bacteria Archaea And Organelles

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Author: Lynne E. Maquat
Publisher: Academic Press
ISBN: 9780080922065
Size: 79.97 MB
Format: PDF, ePub
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Rna Turnover In Bacteria Archaea And Organelles by Lynne E. Maquat

Original Title: Rna Turnover In Bacteria Archaea And Organelles

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the difference in processing of mRNA between eukaryotes, bacteria and archea. Benefit: Processing of mRNA differs greatly between eukaryotes, bacteria and archea and this affords researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Benefit: Keeps MIE buyers and online subscribers up-to-date with the latest research Offers step by step lab instructions including necessary equipment and reagents. Benefit: Provides tried and tested techniques which eliminate searching through many different sources. Tested techniques are trustworthy and avoid pitfalls so the same mistakes are not made over and over

Rna Protein Interaction Protocols

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Author: Susan R. Haynes
Publisher: Springer Science & Business Media
ISBN: 1592596762
Size: 15.19 MB
Format: PDF, ePub
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Rna Protein Interaction Protocols by Susan R. Haynes

Original Title: Rna Protein Interaction Protocols

In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems. Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field.

Rna Methodologies

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Author: Robert E. Farrell
Publisher: Academic Press
ISBN: 0122496965
Size: 50.63 MB
Format: PDF, Mobi
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Rna Methodologies by Robert E. Farrell

Original Title: Rna Methodologies

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

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